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. 2017 Apr 25;7:1141. doi: 10.1038/s41598-017-01100-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apoplastic fluid containing the secreted PstSCR1 triggers cell death in N. benthamiana in a dose-dependent manner. The infiltration of N. benthamiana was conducted with apoplastic fluid containing processed PstSCR1. (a) Shown is a representative leaf of N. benthamiana infiltrated with apoplastic fluid (with no dilution (600–700 μg/μL), 1:3 and 1:10 dilutions in ddH₂O) from N. benthamiana expressing PstSCR1-FLAG and SP-GFP-FLAG. Four days after apoplastic fluid infiltration, the leaves were examined under normal light and UV exposure. (b) Cell death quantification of infiltrated leaf regions. Pixel intensities were normalized by subtracting background in non-infiltrated zone. Error bars represent standard deviations.