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. 2017 Apr 26;7:1195. doi: 10.1038/s41598-017-01179-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M. smegmatis tolerates high doses of 1 mM NaOCl leading to strongly increased protein S-mycothiolation and depletion of MSH in the thiol-metabolome. (A,B) The M. smegmatis wild-type and the ΔmshC mutant strains were cultivated in HdB minimal medium and exposed to sub-lethal concentrations of 0.5–1 mM NaOCl at an OD₅₀₀ of 0.4. In contrast to the wild type, the ∆mshC mutant was unable to grow with 1 mM NaOCl. (C,D) Protein S-mycothiolation was increased in the wild type after exposure to 0.5–1 mM NaOCl stress as shown using non-reducing MSH-specific immunoblot analysis. (E) Thiol-metabolomics revealed the strong depletion of MSH in the wild type in response to 1 mM NaOCl stress indicating that MSH is used for protein S-mycothiolation. The MSH level decreased from 6.5 to 1.6 ± 0.25 µmol/g rdw after 30 min of NaOCl stress (One-way ANOVA, n = 15, P < 0.0001 for Co/NaOCl). (F) The Cys-levels in the control were calculated as 39.4 ± 1.33 nmol/g rdw in the wild type and 79.9 ± 9.75 nmol/g rdw in the ∆mshC mutant (Unpaired t-test, n = 6, p = 0.0173 for WT/∆mshC at t = 0 min). No significant changes in the Cys levels were measured after NaOCl stress in both strains (One-Way ANOVA, n = 15, P > 0.05 for WT and the mshC mutant Co/NaOCl).