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. 2017 Apr 26;7:1195. doi: 10.1038/s41598-017-01179-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Voronoi treemaps show protein abundance of S-mycothiolated proteins identified in M. smegmatis under NaOCl stress using Orbitrap LC-MS/MS analysis. (A) The treemap legend shows the classification of the M. smegmatis proteome according to TIGRfam annotations. (B) The total spectral counts determine the cell size of each protein identified in the proteome dataset and classified according to TIGRfam. The identified 58 S-mycothiolated proteins are color-coded using an orange-red color gradient based on their Cys oxidation level as quantified by the OxICAT data (Tables 2, S3 and S4). Non-modified proteins are colored in grey and S-mycothiolated proteins that were not identified using the OxICAT approach are shown in pink.