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. 2017 Apr 26;7:1195. doi: 10.1038/s41598-017-01179-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematics of the glycerol catabolism, glycolysis, TCA cycle, glyoxylate cycle and gluconeogenesis in M. smegmatis highlighting NaOCl-sensitive thiol-switches. The reversibly oxidized NaOCl-sensitive enzymes are color-coded in light and dark pink indicating 10% and 20% thiol-oxidation increase under NaOCl stress, respectively. The S-mycothiolated proteins are labelled with an asterisk. The pathways of the glycerol catabolism include the aerobic oxidation of glycerol to dihydroxyacetone-phosphate (DHA-P) and the propane-diol-pathway that are catalyzed by (1) the glycerol kinase (GlpK3 or MSMEG_6759) and glyceraldehyde dehydrogenase (GlpD2), (2) the glycerol dehydrogenase (Adh2 or MSMEG_6242) and dihydroxyacetone kinase complex (DhaKLM) and (3) the B12-dependent glycerol dehydratase (DhaB or MSMEG_1546-49) and propane-1,3-diol-dehydrogenase (MSMEG_6239). DHAP enters the glycolysis, TCA and glyoxylate shunt and gluconeogenesis for energy and biomass production. The gluconeogenesis enzymes include GlpX (fructose-1,6-Bis-phosphatase) and PckA (PEP-carboxykinase), while Pgi (glucose-6-phosphate isomerase), Fba (fructose-bisphosphate aldolase), Gap (glyceraldehyde-3-phosphate dehydrogenase), Pgk (phosphoglycerate kinase), GpmA and MSMEG_0970 (phosphoglycerate mutase) and Eno (enolase) are involved in both glycolysis and gluconeogenesis. The glyoxylate shunt includes the isocitrate lyase (AceA) and malate synthase (GlcB).