Table 1.
MSMEG-ID | Protein | Function | CysSSM peptide | % Ox NaOCl/co |
---|---|---|---|---|
Antioxidant enzymes | ||||
MSMEG_4891 | AhpC | AhpC peroxiredoxin | (K)DFTFVC61(+484)PTEIAAFGK(L) | 6,70 |
MSMEG_2421 | OsmC | OsmC family protein | (R)AVDQVC116(+484)TVGR(T) | 10,49 |
MSMEG_3479 | Tpx | Thiol peroxidase | (K)SVLLNIFPSVDTPVC60(+484)ATSVR(T) | 11,57 |
(K)AASSGATVLC80(+484)VSK(D) | −9,09 | |||
(R)FC93(+484)GAEGIENVTTASAFR(S) | 6,91 | |||
Protein biosynthesis and quality control | ||||
MSMEG_1436 | RplC | 50 S ribosomal protein L3 | (R)RPGSIGGC154(+484)ATPGR(V) | 7,32 |
MSMEG_1521 | RpsM | 30 S ribosomal protein S13 | (R)KIEIGC86(+484)YQGLR(H) | 21,77 |
MSMEG_6895 | RpsR2 | 30 S ribosomal protein S18 | (R)VTGNC57(+484)VQHQR(D) | 10,66 |
MSMEG_0839 | Lon1 | ATP-dependent protease Lon | (R)IIDC72(+484)QNLGANR(Y) | 25,70 |
MSMEG_0832 | Def | Peptide deformylase | (R)LFVYDC68(+484)APTR(G) | 5,76 |
Transcriptional regulation | ||||
MSMEG_2750 | IdeR | Iron-dependent repressor IdeR | (R)LLVDVIGLPWEDVHAEAC102(+484)R(W) | — |
MSMEG_4953 | TetR2 | TetR-family transcriptional regulator | (R)LIDAAETC21(+484)LR(A) | — |
MSMEG_0227 | TetR1 | TetR-family transcriptional regulator | (R)LTAILLGPEPGTAC143(+484)R(V) | — |
Biosynthesis of cofactors | ||||
MSMEG_0913 | UmaA | Methoxy mycolic acid synthase 1 | (K)LDLKPGMTLLDVGC76(484)GWGGALER(A) | 10,46 |
MSMEG_6904 | Ino1 | Inositol-3-phosphate synthase | (R)VAIVGVGNC18(+484)ASSLVQGVQYYR(N) | 6,31 |
MSMEG_0793 | ThiG | Thiazole synthase | (R)LGIAALPNTAGC75(+484)R(G) | 10,25 |
Energy metabolism | ||||
MSMEG_6242 | Adh2 | Putative glycerol dehydrogenase | (R)AISEHIQDDWC398(+484)TPGNPR(E) | 4,94 |
MSMEG_6759 | GlpK3 | Glycerol kinase | (K)NGLLTTVC294(+484)YR(L) | 10,73 |
(R)ATLESIC389(+484)YQSR(D) | 5,67 | |||
MSMEG_3086 | TpiA | Triosephosphate isomerase | (R)VAGAADAQEVC192(+484)K(A) | 2,04 |
MSMEG_0911 | AceA | Isocitrate lyase | (K)NGLEPC268(+484)IAR(A) | 11,36 |
MSMEG_5676 | CitA | Citrate synthase | (R)TIDEC143(+484)PTVTAR(F) | 14,23 |
MSMEG_5049 | Kgd | 2-oxoglutarate metabolism enzyme | (R)SSEYC695(+484)TDVAK(M) | 4,60 |
Metabolism of fatty acids and phospholipids | ||||
MSMEG_5639 | EchA6 | Enoyl-CoA hydratase | (R)NALNC26(+484)ELVDSLR(E) | 4,73 |
MSMEG_0531 | MSMEG_0531 | Acyl-CoA dehydrogenase | (R)AAYEYALDYAC285(+484)QR(E) | 4,50 |
MSMEG_6208 | MSMEG_6208 | Acyl-CoA thioesterase | (R)DGDVFC21(+484)IREPEPNTIER(L) | — |
MSMEG_1813 | AccD5 | Propionyl-CoA carboxylase beta chain | (R)VEGRPVGIVANQPTQFAGC356(+484)LDINASEK(A) | 11,49 |
MSMEG_4329 | AccD6 | Acetyl/propionyl-CoA carboxylase | (R)LGGC294(+484)LNSESAEK(S) | 12,96 |
Metabolism of nucleotides | ||||
MSMEG_2299 | NrdE2 | Ribonucleoside-diphosphate reductase | (K)ITHSNLC380(+484)SEILQVSTPSEFNDDLSYAK(V) | — |
MSMEG_1602 | GuaB | Inosine-5′-monophosphate dehydrogenase | (K)VGVGPGSIC325(+484)TTR(V) | 19,34 |
MSMEG_3634 | GuaB2 | Inosine-5′-monophosphate dehydrogenase | (K)VGVGPGAmC302(+484)TTR(M) | 33,37 |
MSMEG_2656 | Pnp | Polyribonucleotide nucleotidyltransferase | (K)ALC248(+484)AAQQELADR(A) | 5,67 |
The M. smegmatis wild type was exposed to 1 mM NaOCl for 30 min and 58 S-mycothiolated proteins were identified using shotgun LC-MS/MS analysis using the Scaffold proteome software based on the mass increase of 484 Da (+MSH) at Cys peptides. The table lists for 29 selected mycothiolated proteins the MSMEG-ID, the protein name, function and the S-thiolated Cys peptide sequence. The OxICAT data were extracted from Tables S3 and S4 for the S-mycothiolated Cys peptides. The full table of the 58 S-mycothiolated proteins is presented in Table S1A,B.