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. 2017 Apr 26;7:1195. doi: 10.1038/s41598-017-01179-4

Table 1.

Selected S-mycothiolated proteins of Mycobacterium smegmatis wild type and quantification of their % oxidation by OxICAT.

MSMEG-ID Protein Function CysSSM peptide % Ox NaOCl/co
Antioxidant enzymes
MSMEG_4891 AhpC AhpC peroxiredoxin (K)DFTFVC61(+484)PTEIAAFGK(L) 6,70
MSMEG_2421 OsmC OsmC family protein (R)AVDQVC116(+484)TVGR(T) 10,49
MSMEG_3479 Tpx Thiol peroxidase (K)SVLLNIFPSVDTPVC60(+484)ATSVR(T) 11,57
(K)AASSGATVLC80(+484)VSK(D) −9,09
(R)FC93(+484)GAEGIENVTTASAFR(S) 6,91
Protein biosynthesis and quality control
MSMEG_1436 RplC 50 S ribosomal protein L3 (R)RPGSIGGC154(+484)ATPGR(V) 7,32
MSMEG_1521 RpsM 30 S ribosomal protein S13 (R)KIEIGC86(+484)YQGLR(H) 21,77
MSMEG_6895 RpsR2 30 S ribosomal protein S18 (R)VTGNC57(+484)VQHQR(D) 10,66
MSMEG_0839 Lon1 ATP-dependent protease Lon (R)IIDC72(+484)QNLGANR(Y) 25,70
MSMEG_0832 Def Peptide deformylase (R)LFVYDC68(+484)APTR(G) 5,76
Transcriptional regulation
MSMEG_2750 IdeR Iron-dependent repressor IdeR (R)LLVDVIGLPWEDVHAEAC102(+484)R(W)
MSMEG_4953 TetR2 TetR-family transcriptional regulator (R)LIDAAETC21(+484)LR(A)
MSMEG_0227 TetR1 TetR-family transcriptional regulator (R)LTAILLGPEPGTAC143(+484)R(V)
Biosynthesis of cofactors
MSMEG_0913 UmaA Methoxy mycolic acid synthase 1 (K)LDLKPGMTLLDVGC76(484)GWGGALER(A) 10,46
MSMEG_6904 Ino1 Inositol-3-phosphate synthase (R)VAIVGVGNC18(+484)ASSLVQGVQYYR(N) 6,31
MSMEG_0793 ThiG Thiazole synthase (R)LGIAALPNTAGC75(+484)R(G) 10,25
Energy metabolism
MSMEG_6242 Adh2 Putative glycerol dehydrogenase (R)AISEHIQDDWC398(+484)TPGNPR(E) 4,94
MSMEG_6759 GlpK3 Glycerol kinase (K)NGLLTTVC294(+484)YR(L) 10,73
(R)ATLESIC389(+484)YQSR(D) 5,67
MSMEG_3086 TpiA Triosephosphate isomerase (R)VAGAADAQEVC192(+484)K(A) 2,04
MSMEG_0911 AceA Isocitrate lyase (K)NGLEPC268(+484)IAR(A) 11,36
MSMEG_5676 CitA Citrate synthase (R)TIDEC143(+484)PTVTAR(F) 14,23
MSMEG_5049 Kgd 2-oxoglutarate metabolism enzyme (R)SSEYC695(+484)TDVAK(M) 4,60
Metabolism of fatty acids and phospholipids
MSMEG_5639 EchA6 Enoyl-CoA hydratase (R)NALNC26(+484)ELVDSLR(E) 4,73
MSMEG_0531 MSMEG_0531 Acyl-CoA dehydrogenase (R)AAYEYALDYAC285(+484)QR(E) 4,50
MSMEG_6208 MSMEG_6208 Acyl-CoA thioesterase (R)DGDVFC21(+484)IREPEPNTIER(L) —
MSMEG_1813 AccD5 Propionyl-CoA carboxylase beta chain (R)VEGRPVGIVANQPTQFAGC356(+484)LDINASEK(A) 11,49
MSMEG_4329 AccD6 Acetyl/propionyl-CoA carboxylase (R)LGGC294(+484)LNSESAEK(S) 12,96
Metabolism of nucleotides
MSMEG_2299 NrdE2 Ribonucleoside-diphosphate reductase (K)ITHSNLC380(+484)SEILQVSTPSEFNDDLSYAK(V)
MSMEG_1602 GuaB Inosine-5′-monophosphate dehydrogenase (K)VGVGPGSIC325(+484)TTR(V) 19,34
MSMEG_3634 GuaB2 Inosine-5′-monophosphate dehydrogenase (K)VGVGPGAmC302(+484)TTR(M) 33,37
MSMEG_2656 Pnp Polyribonucleotide nucleotidyltransferase (K)ALC248(+484)AAQQELADR(A) 5,67

The M. smegmatis wild type was exposed to 1 mM NaOCl for 30 min and 58 S-mycothiolated proteins were identified using shotgun LC-MS/MS analysis using the Scaffold proteome software based on the mass increase of 484 Da (+MSH) at Cys peptides. The table lists for 29 selected mycothiolated proteins the MSMEG-ID, the protein name, function and the S-thiolated Cys peptide sequence. The OxICAT data were extracted from Tables S3 and S4 for the S-mycothiolated Cys peptides. The full table of the 58 S-mycothiolated proteins is presented in Table S1A,B.