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. 2017 Apr 26;7:1179. doi: 10.1038/s41598-017-01325-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamic changes of HMGB1 content in brain and plasma in pilocarpine-induced acute status epilepticus. The content of HMGB1 in the cerebrum was measured by Western blotting 4 h after anti-HMGB1, control IgG or PBS injection. The β-actin was used as a reference protein. Representative results are shown for each group of 2–3 mice (a). The brain levels of HMGB1 are shown as the means ± SEM of 9 mice. *p < 0.05, **P < 0.01 compared with the sham control, ^&&p < 0.01 compared with the PBS control, ^#p < 0.05 compared with the control IgG group (b). Plasma levels of HMGB1 were determined by ELISA at 4 h after onset of seizure . The results are shown as the means ± SEM of 9 mice. *p < 0.05 compared with the sham control, ^&p < 0.05 compared with the PBS control, ^#p < 0.05 compared with the control IgG group (c).