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. 2017 Apr 26;7:1179. doi: 10.1038/s41598-017-01325-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination of the expression of inflammation-related molecules by quantitative real-time PCR in the hippocampus (a) and cerebral cortex (b) in mice with pilocarpine-induced acute status epilepticus. Anti-HMGB1, control IgG or PBS was administered intravenously when mice reached Racine stage 5 after pilocarpine. Brain samples were collected 24 h after antibody administration. The results were normalized to the expression of GAPDH and are expressed as the means ± SEM of 11 mice. *p < 0.05, **p < 0.01 compared with the sham group. ^&p < 0.05, ^&&P < 0.01 compared with the PBS control. ^#p < 0.05, ^##p < 0.01 compared with the control IgG group (a,b).