Figure 9.

A schematic of the experimental design, drug doses and subsequent processes. Protocol #1: Racine stage 5 seizures were induced by pilocarpine which were then treated with PBS, control IgG or anti-HMGB1 mAb for 4 h, then sacrificed, and examined for the BBB permeability, the translocation of HMGB1 (a,b,c), and the apoptosis cells under acute status epilepticus (d). Protocol #2: Mice were administered 10, 50, or 100 μg of human recombinant HMGB1 for 4 h under status epilepticus condition, then BBB permeability was determinated by Evans blue again. Protocol #3: Epileptic mice were treated with PBS or control IgG or anti-HMGB1 mAb for 24 h, then subjected to RT-PCR analyses. Protocol #4: Mice were injected with pilocarpine and either PBS, control IgG or anti-HMGB1 mAb, then immediately subjected to a seizure latency test, followed by a behavior test under 1 h post-treatment. Protocol #5: Mice were pretreated with PBS, control IgG or anti-HMGB1 mAb for 4 h before pilocarpine induction followed by behavior and latency test in 1 hour.