Figure 2.

Reduced release of granule-associated mediators from mast cells lacking SLC10A4 after IgE/antigen-induced activation. (A–E) Wild type (white) and Slc10a4 ^−/− (grey) BMMCs were sensitized with IgE anti-TNP and treated with either OVA-TNP (IgE/ag), Ca²⁺-ionophore (A23187) or vehicle alone (Veh.). The data were obtained from three individual BMMC cultures from two mice per group, each treatment was performed in triplicates and analysed in quadruplicates. The data shown are presented as mean ± SEM. (A) β-hexosaminidase in the cell fraction and supernatants were analysed after one hour activation and the percent release calculated. (B) The amount of histamine from a million cells released into the supernatants. The data shown in (A,B) are from one representative experiment out of three. (C) Tryptase activity in the supernatants was analysed after one hour incubation. The data shown are from one representative experiment out of five. (D) The amount of released IL-6 from a million cells was quantified by ELISA after 18–24 hours of incubation. The data shown are from one representative experiment out of four. (E) The amount of released prostaglandin D2 (PGD2) from a million cells was quantified by ELISA after one hour incubation. The data shown are from one representative experiment out of three. Asterisks mark significant differences (ns, non-significant, **P < 0.01, ***P < 0.001), Student’s t-test.