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. 2017 Apr 28;7:1253. doi: 10.1038/s41598-017-01324-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Actin cytoskeleton on soft matrix (12 kPa). (A) Confocal images of WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts on soft matrix (12 kPa) close to physiological muscle stiffness and stained for F-actin (phalloidin, red) and non-muscle myosin 2 A (NM-2A, green). Nuclei are stained with DAPI (blue). Scale bar: 10 µm. (B,C) Zoom-in of actin cytoskeleton at the cell periphery (B) and in the perinuclear regions (C). In C, confocal images are taken at the apical and basal surface of the cell. Scale bar: 5 µm. (D) Supranuclear actin cable number in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts. Values are means ± SEM; ***p < 0.001 compared with WT. (E) Nuclear thickness in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts. Values are means ± SEM; **p < 0.01 compared with WT. (F) mRNA expression of MYH9 gene expression coding for NM-2A in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts. Values are means ± SEM; **p < 0.01, *p < 0.05 compared with WT.