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. 2017 Apr 28;7:1253. doi: 10.1038/s41598-017-01324-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of ROCK and MLCK inhibition on actin cytoskeleton in myoblasts on soft matrix (12 kPa). (A,F) Confocal images of WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts on soft matrix and stained for F-actin (phalloidin, red) and NM-2A (green) after treatment with the ROCK inhibitor Y-27632 (A), and the MLCK inhibitor ML7 (F). Nuclei are stained with DAPI (blue). Scale bar: 10 µm. Images 1 and 2 show higher magnification of the perinuclear and periphery zones respectively. Zoom-in scale bar: 5 µm. (B,C) Supranuclear actin cable number in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts after treatment with Y-27632 or ML7. Values are expressed as absolute numbers (B) and as percent of baseline values for each cell line (C). (D,E) Nucleus thickness in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts after treatment with Y-27632 or ML7. Values are expressed as absolute numbers (B) and as percent of baseline values for each cell line (C). In (B–E), values are means ± SEM; ***p < 0.001 compared with WT (B,D) or compared to values before treatment (C,E). Only significant difference is figured.