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. 2017 Apr 28;7:1253. doi: 10.1038/s41598-017-01324-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FHOD1 expression in myoblasts on soft matrix (12 kPa). (A) Confocal images of WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts on soft matrix and stained for F-actin (phalloidin, red) and FHOD1 (green). Nuclei are stained with DAPI (blue). (B) Representative western-blot of FHOD1 in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts cultured on soft substrates. Histogram represents FHOD1 quantifications obtained in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts and normalized by GAPDH. Values are means ± SEM, n = 6 in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts; *p < 0.05. Only significant difference is figured; au: arbitrary units. (C) Histogram represent mRNA concentrations of FHOD1, DIAPH1 and DIAPH3 normalized to β2 microglobulin expression and expressed in arbitrary units (au). Values are means ± SEM, n = 5 in WT, Nespr-1^ΔKASH and LMNA^ΔK32 myoblasts, ***p < 0.001 compared with WT. Only significant difference is figured.