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. 2017 Apr 27;7:1229. doi: 10.1038/s41598-017-01229-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HL-60 cells were treated with ATRA for 7 days, after which respiratory burst was induced with PMA in the presence or absence of DPI. Culture medium was collected at the indicated time points, and extracellular metabolites were measured. We performed intragroup ANOVA analysis over the four time points to identify significant changes in metabolite levels. Data are plotted as the mean ± SD (n = 3/group). ANOVA with **p < 0.01 and ****p < 0.0001. The data points indicated with an “x” in the top-right panel were below the metabolite’s limit of detection.