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. 2017 Apr 21;7:1069. doi: 10.1038/s41598-017-00917-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Affinity of Smon0123 with unsaturated GAG disaccharides. (A) DSF analysis. Upper, fluorescence profile of Smon0123 with C∆0S (green: 0 mM and cyan: 1 mM) or 1 mM N,N′-diacetylchitobiose (red). Middle, negative derivative curve plot derived from the fluorescence profile. Lower, the values of T _open and T _closed in the presence or absence of various unsaturated GAG disaccharides. (B) Fluorescence spectrum analysis. Upper, wavelength-scanned fluorescence intensity of Smon0123 with C∆0S (blue: 0 μM; red: 0.25 μM; green: 0.50 μM; purple: 0.75 μM; cyan: 1.0 μM; orange: 2.0 μM; lilac: 5.0 μM; pink: 10 μM; and olive: 20 μM). Middle, the relative fluorescence intensity by addition of increasing ligand concentrations was plotted after modification based on volume change in the cuvette. K _d was determined using the least-squares method. Lower, dissociation constants of Smon0123 with various unsaturated GAG disaccharides. Chitobiose, N,N′-diacetylchitobiose.