Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 21;7:1042. doi: 10.1038/s41598-017-01183-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of hypothyroidism on puberty onset and the reproductive markers of the HPG axis in female mice. (a) Effect of PTU administration on puberty onset. Female mice aged 20 days were induced to hypothyroidism by 0.1% PTU administration in drinking water. The timing of puberty was assessed by monitoring vaginal opening (VO). Data are shown as the box-and-whisker plots with the smallest value and up to the largest (Min to Max). To measure the changes in HPG markers, brain and blood samples were collected at day 34. (b–d) Gene expression change in the hypothalamic regulators, GnIH (b), Kiss1 (c) and GnRH (d). mRNA levels were determined by RT-qPCR relative to the reference gene GAPDH. (e,f) Changes in LH (e) and E2 (f) levels were measured in serum samples. Each column and vertical line represents mean ± SEM. (a) n = 10 in control and n = 9 in PTU group; (b–d) n = 7 per group; (e) n = 12 in control and n = 9 in PTU group; (f) n = 13 in control and n = 14 in PTU group). *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired t test.