Skip to main content
. 2017 Apr 21;7:1042. doi: 10.1038/s41598-017-01183-8

Figure 2.

Figure 2

Effect of hypothyroidism on pubertal onset in GnIH-KO female mice. (a) Schematic diagram of the GnIH precursor DNA and mRNA. GnIH-KO mice were generated by deleting the exon 2 region encoding two kinds of putative mouse GnIH peptides, RFRP1 and 3. SP, signal peptide. (b) GnIH mRNA levels in the hypothalamus of WT or GnIH-KO female mice. GnIH mRNA was not detected in GnIH-KO mice. ND, not detected. (c) WT or GnIH-KO mice aged 20 days were induced to hypothyroidism by 0.1% PTU administration, and the timing of pubertal onset was assessed as described in Fig. 1a. Data are shown as the box-and-whisker plots with Min to Max (c) n = 8 in control and n = 17 in PTU of WT group; n = 10 in control and n = 15 in PTU of GnIH-KO group). **p < 0.01 by two-way ANOVA Bonferroni post-test, ANOVA summary (interaction, F 1,46 = 6.711, p = 0.0128; effect for GnIH expression, F 1,46 = 0.08374, p = 0.7736; effect for PTU administration, F 1,46 = 3.4934, p = 0.0680).