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. 2017 Apr 21;7:1042. doi: 10.1038/s41598-017-01183-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GnIH neurons express TRα and TRβ in the DMH. (a,b) Double staining of in situ hybridization (ISH) for TRα (a) or TRβ (b) and immunohistochemistry (IHC) for GnIH-ir cells were performed with female mice aged 20 days, and the merged image of ISH and IHC was shown. Arrows indicate GnIH cells expressing TRα (a) or TRβ (b). Scale bar indicates 50 μm.