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. 2017 Apr 28;7:1280. doi: 10.1038/s41598-017-01480-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting analysis of NF-κΒ, IKKα and IκΒα expression in four colorectal cancer cell lines, HCT106, HCT116, LoVo, and CT26, which were treated with different combinations of drugs. β-Actin was used as a loading control. The numbers represent different treatment groups: 1: control; 2: Taxol; 3: KLT; 4: Taxol+KLT; 5: Taxol&KLT; and 6: KLT+Taxol. (A) NF-κΒ expression in nuclear; (B) NF-κΒ expression in cytoplasm; (C) NF-κΒ expression in whole cell lysate; (D) IKKα and IκΒα expression in cytoplasm; (E–H). The densitometric analysis bar diagram of NF-κΒ, IKKα and IκΒα expression in the four colorectal cancer cell lines. Where NF-κΒ-c represents NF-κΒ expression in cytoplasm, NF-κΒ-n represents NF-κΒ expression in nuclear, and NF-κΒ-w represents NF-κΒ expression in whole cell. (E) HCT106; (F) HCT116; (G) LoVo; (H) CT26.