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. 2017 Apr 26;7:1210. doi: 10.1038/s41598-017-01264-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding of TGF-β1 to immobilized TβR-II and TβR-I after pre-incubation with tetrameric GAG derivatives. (A) Relative binding of 40 nM TGF-β1 to TβR-II alone and after pre-incubation with 0.1, 2, 20 and 200 µM D.U. of HA dp4 and psHA dp4. (B) Relative binding of 120 nM TGF-β1 to TβR-I alone and after pre-incubation with 0.1, 2, 20 and 200 µM D.U. of HA dp4 and psHA dp4. Values represent the mean ± SD of n = 3. Two-way ANOVA: *p < 0.05; ***p < 0.001 vs. respective treatment; ^#p < 0.05; ^###p < 0.001 vs. TGF-β1 only.