Figure 3.

Multiple alignment of predicted flp sequences. Sequence logo representation representation⁷⁰ of a Flp pilin sequence position weight matrix (PWM), depicting the canonic Flp pilin motif (GXXXXEY and a stretch of hydrophobic amino acids). (A) Multiple alignment of Flp pilin sequences identified by the PWM in various BALO’s with the flp pilin motif indicated by a red outline. (B) Flp2 sequences are truncated forms of Flp pilins, both the N terminus proximal glycine (G) and the amino acid that follows it are missing, creating motif: MXXEY and a stretch of hydrophobic amino acids. The protein logo was created with MEME⁶⁰. Multiple alignments were calculated using GLAM2^{60, 71}. The Flp and the Flp2 multiple alignments were aligned to each other using the Compass program version 2.45⁷², with the similar regions indicated by the ‘=’ marks in between the multiple alignments. Lower case letters are regions not found to be significantly aligned by GLAM2. BALO designations are as in Fig. 2.