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GDF15 KO mice have increased levels of apoptotic cells in kidney (A,B) and heart tissues (C,D). Formalin fixed, paraffin embedded sections of GDF15 WT, TG and KO mice treated with LPS were stained for the TUNEL assay. The number of stained cells were counted for each slide across ten sections at 200 X magnification and averaged. (A,C) are representative images. (B,D) are summarized data. n = 4;*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
