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. 2017 Apr 21;7:1037. doi: 10.1038/s41598-017-00902-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inflammatory gene expression in kidney and heart tissue. WT, TG and KO mice were i.p injected with LPS. RNA was extracted from the cortex of the kidney and heart 16 h after LPS treatment. qRT-PCR was performed using SYBR green to detect the expression of IL-6, TNF-α, KC and MCP-1 and the housekeeping gene GAPDH was used as the internal control. Data is expressed using the ∆∆CT method and normalized to WT + LPS. (A) Kidney tissues (B) Heart tissues. n = 3–5 = ;*P ≤ 0.05.