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. 2017 Apr 25;7:1143. doi: 10.1038/s41598-017-01247-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results shown for systems labelled by the points in the phase diagram (Fig. 1): (8) Chol20₃₁₆ (L_o phase), (5) Chol10₃₂₆ (L_d phase), (1) Chol10₃₀₁ (gel), (3) Chol10₃₁₆ (ordered/disordered/hexagonal). First row: Deuterium order parameter distributions along the sn-2 chain of DPPC. Available experimental data are shown in white (full line): For the Chol20₃₁₆ system (8), given here are the data measured for the L_o phase (DPPC + 40 mol-% cholesterol at 308 K)⁵¹. For the Chol10₃₂₆ system (5), the experimental data are for the L_d phase system (pure DPPC at 314 K)⁵². Second row: The spatial correlation of lipid chain order (sn-2 chain of DPPC) between the leaflets. Third row: In-plane lipid displacement map. Fourth row: Thickness maps.