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. 2017 Apr 21;7:1075. doi: 10.1038/s41598-017-00949-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) A schematic representation of a glass backed 35 mm petri dish with three agarose drops containing chemokines, and one agarose drop containing PBS as control. Cells migrate under the agarose spot in response to the chemokines. (B) A schematic representation of chemotactic gradient established within the agarose spot (C) A schematic representation of glass back Petri dish with agarose drops from the top, dotted line and writing on the bottom of the Petri dish are also shown.