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. 2017 Apr 21;7:1075. doi: 10.1038/s41598-017-00949-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–C) Diffusion of Cascade Blue dextran (mean MW = 10 kDa) from an agarose spot at different time intervals time. The edge of the spot is shown by dotted white line. (D) Fluorescence intensity along a central strip of the agarose spot (shown as a dotted yellow line) at different time intervals. The edge of the spot is shown by dotted grey line. (E and F) Diffusion of Fluorescein dextran (mean MW = 40 kDa) from an agarose spot at 0 and 30 minutes time intervals. (G) Fluorescence intensity along a central strip of the agarose spot at different time intervals. The edge of the spot is shown by dotted grey line.