Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 24;7:1081. doi: 10.1038/s41598-017-01167-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Genome-wide transcriptional analysis of microRNA expression in eCig treated NHBE. (A) Treatment with any eCig liquid has a significant effect on miRNA expression. Shown here is a dendrogram representing hierarchical clustering of samples when comparing expression of all 2541 miRNAs. The clusters were generated using Euclidean distance and average linkage clustering. Samples completely segregated based upon treatment with any eCig liquid (black bar). Individual treatment groups were untreated control (CTL), cigarette smoke condensate (CSC), non-vaporized liquid without (LIQ-NONIC) or with (LIQ-NIC) nicotine, and vaporized/condensed liquid without (VAP-NONIC) or with (VAP-NIC) nicotine. (B) SAM-Seq identified 578 miRNA significantly differentially expressed between samples with any eCig liquid treatment (n = 12), when compared to controls (n = 3). Shown are normalized expression levels for these 578 selected miRNA. Rows represent miRNA and columns represent samples. Blue indicates low expression, while yellow indicates high expression. (C) SAM-Seq identified 125 microRNA as significantly differentially expressed between cells treated with vaporized liquid (n = 6) compared to non-vaporized liquid (n = 6). Shown are normalized expression levels for these 125 selected miRNA. Rows represent miRNA and columns represent samples. Blue indicates low expression, while yellow indicates high expression.