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. 2017 Apr 26;7:1166. doi: 10.1038/s41598-017-00973-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of Mmp13 expression by Etv4 and Etv5. (A) FAIRE-seq and ChIP-seq data at the matrix metalloproteinase 13 (Mmp13) gene locus as a target of Etv4 and 5. Data are shown as in Figs 2A and 4E. Arrow heads show the positions evaluated by FAIRE-qPCR in (D). (B) RNA-seq data of siEtv4/5-transfected EpRas cells treated with TGF-β. siNC; negative control siRNA. Error bars, 95% confidence intervals. (C) Matrigel invasion assay in EpRas cells. Cells were transfected with the indicated siRNAs and then seeded on the Matrigel-coated plate, incubated for 48 h with TGF-β, and fixed. (upper panels) Representative images of the cells that migrated through the Matrigel-coated membrane. (bottom graph) Quantified data representing the means of four independent experiments. Error bars, standard deviations. (D) Changes in the FAIRE-seq signal intensities by Etv4/5 siRNAs at FAIRE-positive regions of Mmp13 gene locus shown in (A). EpRas cells transfected with the indicated siRNAs were treated with TGF-β for 4 days and fixed for FAIRE-seq data acquisition. The data represent the result of two biological replicates. Error bars, standard deviations.