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. 2017 Apr 25;7:1160. doi: 10.1038/s41598-017-01294-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of adipogenic gene expression in the three tissues-derived MSCs by RT-PCR. UC-MSC, Ad-MSC and BM-MSC were incubated with or without adipogenic medium for 14 days. (A) Adipogenic markers including PPARγ, FABP4, PLIN1 and LPL were detected at the indicated days of differentiation. Actin was used as a loading control. The picture was cropped and adjusted using photoshop software and the original image was presented in Supplementary Fig. 1. (B–E) The quantification of the four marker genes was performed using Gel-Pro software and presented. (F) The correlation of adipogenic capacity with PPARγ expression in the three sources-derived MSCs was performed using linear regression analysis. *P < 0.05 and **P < 0.01, Student’s t-test.