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. 2017 Apr 24;7:1106. doi: 10.1038/s41598-017-01315-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in transcriptome and amino acid profiles in hBM-MSCs stored in PBS for 6 and 12 h. (a) Heat map of genes with altered expression; 34 genes related to amino acid metabolism were differentially expressed at 6 and 12 h according to a microarray analysis. Red and green areas indicate up- and downregulated genes, respectively. (b) Star plot showing altered levels of 17 amino acids after 6 and 12 h of incubation in hBM-MSCs. Data are based on percentage mean composition levels of 17 amino acids after normalization to the corresponding values in the control group. Three independent experiments were performed. Selected-ion monitoring (SIM) chromatograms of GABA, lysine, and tyrosine in (i) 0 h control (ii) 6 h group (iii) 12 h group. IS, Internal standard (norvaline).