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. 2017 Apr 28;7:1251. doi: 10.1038/s41598-017-01133-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene fitness analysis during in vitro growth of C. jejuni M1cam, 11168 and 81–176. (a) Rarefaction analysis of intragenic Tn insertions. (b) Density plot fitness score (Log₂ fold-change measured reads/expected reads) per gene. Dots indicate fitness score cut-off values. (c) Overlap of homologous gene (HG) clusters (HGs) required for fitness in C. jejuni M1cam, 11168, and 81–176, representing the “pan-fitness genome”. HG clusters that are required for fitness in one or more strains are displayed. (d) Functional class (COG; Cluster of Orthologous Genes) enrichment analysis of fitness genes. Fisher exact test with Q-value multiple testing correction; *Q < 0.05, **Q < 0.01 and ***Q < 0.001.