Figure 2.

Identification of conditional essential genes in C. jejuni. (a) Effect of Tn insertions on the ability of C. jejuni M1cam to colonize commercial broiler chickens, infect gnotobiotic piglets, adhere and invade gut epithelial tissue culture cells, survive in flies and at 4 °C in various media (chicken juice, BHI, tap water, rain water, and sterile water). Genes of which Tn mutants showed significantly attenuated or enriched fitness in the experimental models are shown and are grouped according to their COG functional classification. Data represented as Log₂ fold-change is also presented in Table S4. (b) Gene-model interaction network showing attenuated (orange lines) and enriched (blue lines) Tn-seq scores linked to their respective models; the thickness of the connecting lines corresponds to the Log₂ fold-change (input/output). The gene numbers correspond to the C. jejuni M1cam locus-tags¹³ and are color-coded according to their COG functional class. The size of the genes and the models displayed increases with the number of interactions.