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. 2017 Apr 28;7:1251. doi: 10.1038/s41598-017-01133-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of Tn mutant library screen during survival at low temperature under nutrient-rich and –poor conditions. (a–e) Survival of defined C. jejuni M1cam gene deletion mutant in (a) BHI, (b) chicken juice, (c) sterile water, (d) tap water, (e) rain water. Survival of trxC defined gene deletion mutant and complemented mutant (F), at 4 °C in different media. Data shown are Log₁₀ decrease of CFU/ml relative to the 0 day time-point and plotted as means with SEM (n ≥ 4). Statistical significance was analysed using a Mann-Whitney test with *P < 0.05, **P < 0.01 and ***P < 0.001.