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. 2017 Apr 28;7:1251. doi: 10.1038/s41598-017-01133-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of Tn mutant library screen during adhesion and invasion of human gut epithelial tissue culture cells. Adhesion to (a), and invasion (b) of, Caco-2 cells by C. jejuni M1cam defined gene deletion mutants. Mutants in luxS and pstA, which were not identified in our Tn-seq screen with Caco-2 cells, were included as negative controls and rpoN, which was previously shown to be required for adhesion and invasion^{81, 82}, served as a positive control. Caco-2 adhesion (c), and invasion (d), of gene deletion and genetically complemented mutants. Data is represented as percentage of wild-type (n ≥ 3) and plotted as means and SEM. The competitive index (CI) was calculated by dividing the ratio of mutant to wild-type bacteria recovered upon (e) adhesion to, and (f) invasion of, Caco-2 cells by the ratio of mutant to wild-type bacteria that were used in the inoculum. Statistical significance was calculated using a Mann-Whitney test where *P < 0.05, **P < 0.01 and ***P < 0.001.