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. 2017 Apr 26;7:1172. doi: 10.1038/s41598-017-01178-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of AKAP7 splice variants and ITGA3 on differentially adherent lymphocyte populations. (A) Actively proliferating primary human lymphocytes following 72 hours of in vitro stimulation with PHA. (B) Adherent fraction of lymphocytes bound to extracellular matrix rich in ITGA3 ligands following 20 minutes of serum starvation. (C) Non-adherent fraction of lymphocytes collected following 20 minutes of serum starvation. (D) Transcriptional expression levels of total ITGA3 and AKAP7 splice variants in adherent and non-adherent lymphocyte fractions as measured by qRT-PCR. Splice variants which are translated to produce PKA-binding AKAP7 isoforms are highlighted. Expression levels are presented as fold difference relative to the adherent fraction and were statistically compared using two-sample two-way paired t-test.