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. 2017 Apr 25;7:1126. doi: 10.1038/s41598-017-01262-w

Figure 1.

Figure 1

Effects of Chlamydia spp. on sperm motility and viability. Human sperm motility (%) after 6 h of in vitro incubation without bacteria (Control), or with increasing concentrations of EBs of CT serovar E (a) or serovar LGV (b) per million spermatozoa. Human sperm viability (%) after 6 h of in vitro incubation without bacteria (Control), or with increasing concentrations of EBs of CT serovar E (c) or serovar LGV (d) per million spermatozoa. (e) Sperm membrane integrity (%) in human spermatozoa after in vitro incubation without bacteria (Control), or with increasing concentrations of EBs of CT serovar E or serovar LGV per million spermatozoa. (f) Murine sperm viability (%) after 30 min of in vitro incubation without bacteria (Control) or with increasing concentrations of EBs of C. muridarum per million spermatozoa. As positive controls, sperm fractions were incubated with uropathogenic E. coli (1 × 106 CFU/mL). Data are shown as mean ± SD. Fractions of human (n = 54) and mouse (n = 24) sperm samples were tested separately and maintained at 37 °C throughout all procedures. Statistical analysis was performed using one-way ANOVA with Bonferroni post hoc test analysis and no significant differences were found in any condition (p < 0.05).