Table 2.
Effects of CT on the mitochondrial physiology and DNA integrity of human sperm.
| Sperm mitochondrial membrane potential, ΔΨm (%)a | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CT | Serovar E | Serovar LGV | ||||||||
| Sample | Vehicle | Control+ | 1 × 105 EBs | 1 × 106 EBs | 1 × 107 EBs | Vehicle | Control+ | 1 × 105 EBs | 1 × 106 EBs | 1 × 107 EBs |
| High ΔΨm | 54.2 ± 3.8 | 45.2 ± 2.9* | 55.0 ± 3.9 | 55.7 ± 8.4 | 51.1 ± 6.9 | 63.3 ± 6.2 | 48.3 ± 6.5* | 67.5 ± 15.5 | 59.7 ± 5.4 | 58.3 ± 6.8 |
| Low ΔΨm | 20.1 ± 6.5 | 7.7 ± 2.2* | 21.1 ± 4.4 | 19.1 ± 8.3 | 23.0 ± 7.8 | 16.2 ± 4.5 | 6.5 ± 2.7* | 12.3 ± 7.0 | 20.0 ± 8.3 | 20.0 ± 7.3 |
| Sperm DNA fragmentation b | ||||||||||
| DNA fragmentation index | 4.0 ± 1.0 | 15.6 ± 0.7* | 4.2 ± 0.6 | 4.3 ± 2.2 | 3.5 ± 2.6 | 3.1 ± 2.6 | 17.1 ± 1.1* | 4.1 ± 1.3 | 5.3 ± 3.6 | 2.9 ± 1.3 |
Percentages of human sperm exhibiting high and low mitochondrial membrane potential (ΔΨm)a, and evaluation of DNA fragmentationb, in human sperm samples after 6 h of in vitro incubation without bacteria (Vehicle), or with increasing concentrations of EBs of CT serovar E or serovar LGV. Data are shown as mean ± SD, n = 36. Statistical analysis was performed using one-way ANOVA with Bonferroni post hoc test analysis. *p < 0.05.