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. 2017 Apr 26;7:1177. doi: 10.1038/s41598-017-01311-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neurosphere formation of iMuSCs. (A) BF image of the control MuSCs that could not form neurospheres. (B) BF image of the iMuSC-formed neurospheres in suspension. (C–F) Immunofluorescence images of cryosectioned neurospheres after 7 days of culture. They stained positive for CNPase (red, C), Nestin (green, D), Nefm (red, E), and Gfap (green, F). Nuclei were stained with DAPI (blue). Scale bar = 100 µm. (F) Whisker plots summarize the gene expression profiles of five biological replicates of iMuSC-formed neurospheres after 7 days of culture compared to undifferentiated iMuSCs by qPCR.