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. 2017 Apr 26;7:1177. doi: 10.1038/s41598-017-01311-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induced neuronal differentiation of iMuSCs. (A–F) Gene expression kinetics were analyzed by qPCR during the 21-day neuronal differentiation period of the plated iMuSC-formed neurospheres. Data are presented as ± SEM of five biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (G,H) Immunocytochemical staining of neurons after 21-day differentiation from iMuSC-formed neurospheres. β-Tubulin III (red, G,H) and AChE (green, H). Nuclei were stained with DAPI. Scale bar = 100 µm (G) and 10 µm (H).