Figure 3.

Effects of GPR139 mutations on pharmacological profiles of 1a and 7c. The data demonstrates that the residues F109^3×33, H187^5×43, W241^6×48 and N271^7×38 are important for GPR139 activation by 1a and 7c, whereas residue E108^3×32 is not. Concentration-response curves of (a) 1a and (b) 7c, on the mutants with an effect (plus WT, mock and E108A^3×32). The graphs are one representative (mean ± S.D.) out of three independent experiments performed in (a) triplicates and (b) duplicates. All responses are normalized to myc-GPR139(WT) (0% = buffer, 100% = 8 μM 1a or 100 μM 7c).