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. 2017 Apr 26;7:1203. doi: 10.1038/s41598-017-01355-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescent imaging of the primary buffalo hepatocytes cultured on collagen-coated and polyHEMA-coated plates on the sixth day at 200x magnification. The nucleus of the primary buffalo hepatocytes were stained with DAPI and the cytoskeletal protein F-actin was stained with Phalloidin-TRITC stain. Hepatocytes formed patches on collagen-coated plates (CH) on the sixth day of the culture. However, different layers of cells could be observed in the intact spheroids formed on the polyHEMA coated dishes on the sixth day (PH).