Table 2.
Culture systems for buffalo hepatocytes.
| Culture system | Type of 3D culture | Media | First three days | Next three days |
|---|---|---|---|---|
| Collagen coated plates with Hepatozyme-SFM media (CH) | 17 µg/cm2 collagen coated* | Hepatozyme-SFM | Media + 10% FBS | Media |
| Collagen coated plates with Heaptozyme-SFM media containing collagen (CHSC) | 17 µg/cm2 collagen coated* | Hepatozyme-SFM | Media + 10% FBS | Media + 166 µg/ml collagen |
| Collagen coated plates with William’s E media (CW) | 17 µg/cm2 collagen coated* | William’s E | Media + 10% FBS | Media |
| Collagen coated plates with William’s E media containing collagen (CWSC) | 17 µg/cm2 collagen coated* | William’s E | Media + 10% FBS | Media + 166 µg/ml collagen |
| polyHEMA coated plates with Hepatozyme-SFM media (PH) | 12 mg/ml polyHEMA coated | Hepatozyme-SFM | Media + 10% FBS | Media |
| polyHEMA coated plates with Hepatozyme-SFM media containing collagen (PHSC) | 12 mg/ml polyHEMA coated | Hepatozyme-SFM | Media + 10% FBS | Media + 166 µg/ml collagen |
| polyHEMA coated plates with Hepatozyme-SFM media containing serum (PHF) | 12 mg/ml polyHEMA coated | Hepatozyme-SFM | Media + 10% FBS | Media + 10% FBS |
| polyHEMA coated plates with William’s E media (PW) | 12 mg/ml polyHEMA coated | William’s E | Media + 10% FBS | Media |
| polyHEMA coated plates with William’s E media containing collagen (PWSC) | 12 mg/ml polyHEMA coated | William’s E | Media + 10% FBS | Media + 166 µg/ml collagen |
| polyHEMA coated plates with William’s E media containing serum (PWF) | 12 mg/ml polyHEMA coated | William’s E | Media + 10% FBS | Media + 10% FBS |
| Hanging drops with Hepatozyme-SFM media (HDH) | Hanging drop | Hepatozyme-SFM | Media + 10% FBS | Media |
| Hanging drops with William’s E media (HDW) | Hanging drop | William’s E | Media + 10% FBS | Media |
*Indicates that those culture systems are more towards 2D rather 3D.