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. 2017 Apr 21;7:1028. doi: 10.1038/s41598-017-01181-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of injected dsRNA in experimental animals. Shrimp (2.618 g ± 0.527 SD, n = 30) injected with 42.85 µg dsRNA were sampled (n = 5) at indicated time points, and dsRNA levels were measured by RT-qPCR using assays with specificity for the injected treatments. Another experiment verified that when using primers nonspecific for the VP28 dsRNA treatment, but with specificity for VP28 mRNA, no Cq-values were obtained through 40 cycles for shrimp in the VP28 dsRNA treatment group. One-way ANOVA did not reveal any significant changes between groups or time points at α = 0.05 (p = 0.255). Error bars indicate the 95% CI around the mean.