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. 2017 Apr 21;7:1028. doi: 10.1038/s41598-017-01181-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histology examination carried out to identify WSSV-specific pathology in experimental animals. Shrimp collected at different time points were sectioned and stained with hematoxylin-eosin for histological examination. All baseline samples were collected from the no treatment (PBS) group prior to challenge at 0 dpi, and all survivor samples were collected at 10 dpi from the VP28 dsRNA treatment group. Moribund shrimp were collected from the GFP dsRNA treatment group during the challenge trial, where infected cells exhibiting hypertrophic nuclei containing eosinophilic inclusion bodies were identified (indicated by black arrows).