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. 2017 Apr 21;7:1028. doi: 10.1038/s41598-017-01181-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR detection of WSSV in experimental animals. DNA isolated from uropods from shrimp collected at different time points before, during and after challenge was subjected to a qPCR assay detecting WSSV VP28. Cq-values from individual shrimp were compared to a standard curve, made up of 10-fold serial dilutions of a plasmid containing a VP28 insertion, to obtain absolute quantities of WSSV genome copy numbers. The Y-axis represents the absolute WSSV genome copy numbers obtained from each individual shrimp sample. Error bars indicate the 95% CI around the mean.