Figure 6.
48 hours after dsRNA injection, relative gene expression on a panel of host genes associated with small RNA silencing was carried out by RT-qPCR assays. Relative gene expression was measured through fold variation from the lowest obtained normalized expression value observed in all samples. One-way ANOVA with Dunnett’s multiple comparisons test was performed individually for each gene comparing the two dsRNA treatment groups to the no treatment (PBS) group. Significant variation at α = 0.05 is indicated and error bars indicate the 95% CI around the mean.