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. 2017 Apr 21;7:1028. doi: 10.1038/s41598-017-01181-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Small RNA length distribution given as fractions of total. RNA was isolated from stomach tissue and equal amounts were pooled for each sample within a given group at a given time point. RNA sequencing was performed on these pools and obtained data was subjected to an analysis of read length distribution frequency. Here, relative distribution of read lengths obtained from reads mapping to the VP28 (a) and GFP (b) dsRNA treatment sequence are shown to emphasize the dominance of 22 nt reads in samples were the only source of such sequences are the injected dsRNA.