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. 2017 Apr 26;7:1171. doi: 10.1038/s41598-017-01238-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) FLIM image of the papillary structure, colors correspond to the values of mean fluorescence lifetime in the 100 ÷ 2000 ps range. (b) segmentation of the FLIM image of papilla into the inner area (red), outer area (black) and intensity mask area (blue). The yellow area corresponds to the melanin-rich area around the papilla. (c–f) – distributions of fast (τ ₁) and slow (τ ₂) decay components, amplitude of the fast component a ₁ and mean fluorescence lifetime, respectively.