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. 2017 Apr 25;7:1157. doi: 10.1038/s41598-017-00786-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Divergence time estimates in amphioxus lineage. (A) Estimate based on nuclear transcriptome comparison calibrated with vertebrate fossil records²⁶. Numerals at nodes denote estimated split time in Ma. (B) Estimate based on mitogenomic comparison with ingroup calibration points derived from (A). Timings of formation of the Isthmus of Panama³⁶ and of Arabia-Eurasia³⁸ and Indo-Asia collisions^{72, 73} are also shown. Blue bars denote 95% confidence interval.