Figure 5.

Sle1 is responsible for the increase autolysis of the clpP mutant. (A) Triton X-100 induced autolysis. The average of two independent experiments were shown. (B) Zymogram of S. aureus murein hydrolase activity. Concentrated culture supernatants from the wild-type (WT) and its derivatives were run on 12% acrylamide-SDS gels containing heat killed S. aureus RN4220 cells. Numbers to the left indicate the molecular weights of size standards. Dark bands indicate regions of murein hydrolase activity. The arrows showed the activity of murein hydrolase Sle1.