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. 2017 May 2;7:1343. doi: 10.1038/s41598-017-01468-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Most highly expressed genes from the CD8+ T cell module were enriched for nearly 12-fold in CD8+ T cell populations relative to bulk samples. The summed expression of the top 50 most expressed genes from each module was averaged across all samples in each cell population and from the bulk samples. Fold change relative to bulk skin is defined as the ratio of the average summed expression across samples in each population to the average summed expression in the bulk samples.